Whole-genome characterization of Rosa chinensis AP2/ERF transcription factors and analysis of negative regulator RcDREB2B in Arabidopsis

Abstract Background Rose ( Rosa chinensis ) is a traditional famous flower with valuable ornamental characteristics. However, drought stress restricts its growth and development, leading to an abnormal phenotype. One of the main transcription factor (TF) protein groups in plant kingdom are APETALA2/ethylene-responsive (AP2/ERF) proteins potentially involved responses various plants. Results Our investigation mainly focused on exploring genome rose thereby we discovered 135 apparent AP2/ERF TFs. Phylogenic analyses revealed that RcAP2/ERF genes categorized into DREB, Soloist, AP2, ERF subfamilies, further classified these 17 groups, same as Malus domestica Arabidopsis thaliana . The analysis gene structure introns ranged from 0 9 number. Pattern examination demonstrated predominantly consists typical AP2 domains, which 2nd motif most ubiquitous. Distributions cis -acting elements indicated members family frequently phytohormone response species. Also, distribution mapping chromosomes class dispersed among all seven chromosomes. Additionally, isolated novel DREB A2 subgroup named it RcDREB2B Subsequently, transcript accumulation was repressed under mild severe root samples rose. targeted nucleus exhibited transactivation yeast cells. overexpression found promote sensitivity higher salt concentration, ABA, PEG at germination post-germination stages. Twelve putative osmotic ABA-related were impaired -overexpressing Conclusions results provide comprehensive information regarding structure, phylogenic, bring insight complex transcriptional regulation Findings this study would also contribute understanding